Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc CHAPS Lysis Buffer (2X) - 50ml
CHAPS Lysis Buffer (2X) - 50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 1L
Tris-HCl Buffer (1 M pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Glycine-Native Running Buffer (10X) - 1L
Tris-Glycine-Native Running Buffer (10X) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-P+ Buffer (10X, pH 7.4) - 4x500ml
HBS-P Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.6) - 1L
Tris Buffered Saline TBS (10X pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MOPS Buffer (1 M, pH 8.0) - 500ml
MOPS Buffer (1 M pH 8 0) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.5) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 7 5) - 2x1L (For Research Use and Further Manufacturing Only)
National Diagnostics TRIS-GLYCINE ELECTROBLOTTING B
Tris-Glycine Electroblotting Buffer (10X), Stable, economical, ultra pure transfer buffer for Western Blotting procedures. 18 megOhm water. 0.2 micron filtration, 1 LT
Boston Bioproducts Inc Succinate Buffer (0.2 M, pH 5.5) - 500ml
Succinate Buffer (0 2 M pH 5 5) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (Sterile-Filtered, RNase Free) - 500ml
Phosphate Buffered Saline PBS (Sterile-Filtered RNase Free) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X) - 500ml
HEPES Buffered Saline (2X) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Paraformaldehyde (4%, PBS with Mg & EGTA) - 250ml
Paraformaldehyde (4% PBS with Mg and EGTA) - 250ml (For Research Use and Further Manufacturing Only)
Growcells TAE BUFFER 50X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3